FLIM Systems for Zeiss LSM 710

This upgrade to the Zeiss LSM 710 multiphoton microscope includes a time-correlated single photon counting (TCSPC) system produced by Becker & Hickl GmbH. The instrument consists of: (i) Simple-Tau 152 TCSPC FLIM: a highly flexible, modular, dual channel parallel acquisition FLIM system; (ii) two HPM-100-40 GaAsP hybrid detector modules, with high count rate, fast response, no after pulsing and no diffusion tail; (iii) Data Analysis Software for Fluorescence Lifetime Imaging. The system allows time-series FLIM as fast as 2 images/second, fluorescence correlation spectroscopy (FCS) recording, single/double/triple-exponential decay analysis, FRET measurement without bleedthrough, double-exponential FRET for separation of interacting and non-interacting protein fractions, and ion concentration measurements.