Thermo Scientific Tundra Cryo-TEM

The Thermo Scientific Tundra Cryo-TEM is a 100kV Transmission Electron Microscope for imaging protein structure. It is equipped with a field-emission gun (X-FEG), high-quality lenses, computer-controlled apertures, and a sensitive Falcon C direct detector. The system also includes a semi-automated loader with a dedicated Cryo-Loading Workstation for subsequent loading of up to five AutoGrids into the microscope without need to open the area for handling and exchange of the vitrified AutoGrids. EPU software controls the microscope and guides the operator through the process of imaging, quality evaluation, and selection of optimal grid areas for single particle analysis. This guidance is designed for users with various levels of expertise, including users with little experience in cryo-EM. The EPU data management platform has 50TB of storage, which will interface with additional backup storage in Brown University’s Center for Computation and Visualization. The microscope can also be used for imaging clipped room-temperature EM grids with ultrathin sections.