Zeiss LSM 780

The Zeiss LSM780 is a state of the art confocal microscope. In addition to routine single-photon confocal imaging, it is equipped with a Coherent Chameleon multi-photon laser for deeper sample penetration, detection of dimmer fluorophores, and reduced photodamage. It is also equipped with a Definite Focus module to eliminate thermal drift or focal offset in the Z-dimension. This microscope is well suited for confocal fluorescent imaging of cells and tissues, viewing samples in culture dishes, culture plates (6 wells and greater), chamber slides that can be inverted and a variety of custom made (by user) chambers no larger than a standard culture plate. Imaging through plastic dishes is limited to 5x, 10x and 20x lens. Higher magnification must be through coverslips on slides or MatTek-type culture dishes. All slides must be sealed, clean and dry prior to placing on the stage. Imaging Modes: Multi-photon imaging Laser scanning fluorescence and DIC (Nomarski) 3-D “Z-series” Time-series Tile scans (mosaics) Co-localization, FRET, FRAP, linear unmixing FCS, RICS FLUORESCENCE EXCITATION/EMISSION 458, 488, 514nm Blue/Green for blue, green, yellow dyes, e.g., CFP,YFP,GFP, Cy2, FITC-like dyes 561 nm Green/Red for Cy3, m-Cherry, or rhodamine-like dyes 633 nm Red/Far-Red nm for far-red dyes like Cy5, TO-PRO, Alexa647 Coherent Chameleon 2-photon laser with tunable range 690-1040 Emission bandwidth for all dyes/laser lines is continuously variable providing huge flexibility for collecting images of unusual dyes and for minimizing bleed-through between colors. Objective Lenses: 5x /.25 Fluor 10x / 0.50 Fluar DIC II 20x / 0.80 Plan-Apochromat DIC II 40x / 1.10 LD C-Apochromat WATER 40x/1.30 Fluar Oil 63x / 1.40 Plan-APOCHROMAT OIL DIC Charges: $49.75 / hour Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar. Contact Tegy, or Jenny, to arrange training. Location LH 704