Leica Stellaris 8 DIVE Multiphoton Confocal System

– A combination of laser scanning confocal microscopy and multiphoton microscopy – Upright Leica DM6 CFI microscope with AFC (Adaptive Focus Control) and XY scanning stage – Confocal Imaging with 4 visible laser lines and 4 tunable HyD detectors (410-850nm) – Multiphoton, Second Harmonic Generation (SHG), and Third Harmonic Generation (THG) imaging using pulsed invisible dual IR lasers: Spectra Physics IR laser Insight X3 DUAL (tunable 700-1300nm & fixed 1045nm beam), dual beam-routing with VBE – Multiphoton imaging with 3 spectrally tunable 4Tune NDD channels (380-800 nm) for simultaneous spectral separation of up to 3 fluorescent signals and sequential acquisition of unlimited signals – Extra-large field of view, focus adapting tile scanning with Navigator – Super-resolution imaging (120 nm XY, <300nm Z) using LIGHTNING adaptive deconvolution – Fluorescence lifetime (FLIM) imaging using a pulsed IR laser, with analysis options including average photon arrival time (TauSense) and Time Correlated Single Photon Counting (TCSPC)-based FLIM via FALCON (FAst Lifetime CONtrast) – FALCON for comprehensive FLIM analysis, such as exponential fitting, FLIM phasor analysis, pattern fitting and FLIM-FRET analysis – Transmitted light detector for bight-field imaging – Resonant scanner for high-speed imaging – FRAP/photostimulation – FLIM-FRET – Okolab laser safety box Confocal: Academic users: $40/h, Industry users: $52.50/h Multiphoton: Academic users: $66/h, Industry users: $120/h – DM6 CFI upright fixed stage microscope – Conventional fluorescence filters and LED light sources for eyepieces observation (DAPI, GFP, mCherry) – Maerzhaeuser motorized platform; Motorized xy positioning and manual height adjustment to accommodate a variety of specimens – Exchangeable nosepiece, including a single objective holder and a motorized 6-position nosepiece Scanners – FOV scanner (10 frames/sec at 512 x 512) – Resonant Scanner (8kHz, 28 frames/sec at 512 x512) Excitation Lasers – 1P