Confocal Microscopes

The Keck Center’s laser-scanning confocal microscopes use laser excitation, motorized scanning mirrors, confocal pinholes, and amplification or photon-counting detectors to collect high-resolution optical sections that exclude out-of-focus light.   Through serial, optical sectioning of fluorescently-labelled samples, these systems are capable of producing high-resolution 3D and 4D datasets. All publications that include data acquired on the Leica SP8X confocal must acknowledge NIH grant S10 OD016240. KLONDIKE scan head electronics, CUDA computer workstation, and LASX Expert software with Lightning deconvolution were funded by the UW Student Technology Fee.