Leica TCS SP8 Confocal Microscope with Environmental Chamber

Laser scanning confocal microscope for optical sectioning, z-stacking and tile scanning. Suitable for fixed and live cells, medium thickness tissue sections and organoids up to ~100 μm thickness. Unique advantages of this microscope include the fast resonant scanning option and interline scanning mode for dynamic/live events and colocalization, as well as flexible spectral detection for fluorescence fingerprinting, unmixing and for unconventional fluorophores. This microscope provides full environmental control with CO2 and humidity for live cells. Modes of operation: Conventional, point-by-point laser scanning Fast resonant scanning for living, motile cells Fluorescence Recovery after Photobleaching (FRAP) Automated mosaic stitching Reflection mode Spatial resolution: ~ 250 nm XY and 600 nm Z Available laser for fluorescence excitation: 405, 442, 458, 488, 514, 561, 633 nm Spectral detection with Acusto-Optical Beam Splitter (AOBS) Sensitive Hybrid Detectors (HyD) Inverted configuration Best for routine imaging of fixed cells and tissues; ideal for live cell confocal imaging due to low dwell reducing phototoxicity and maintaining cell viability.