Leica SP8 DIVE Multiphoton (2-Photon) Microscope

This microscope is preferred for thick tissues and intravital imaging. It enables deep imaging (up to ~500 μm) using infrared long wavelengths and highly focused z-spot illumination for 2- and 3-photon excitation and non-descanned detectors for efficient detection. Compared to the visible light, the infrared light penetrates deeper into tissues with increased imaging depth, minimal light scattering and reduced phototoxicity. The 2- and 3-photon effect provides high z-resolution without needing a confocal pinhole. Modes of operation Conventional galvo point scanning for highest resolution 8 kHz resonant scanning for highest speed (28 fps at 512 x 512) Non-linear optical imaging by second and third harmonics (SHG/THG) SpectraPhysics Insight X3 dual near infrared laser: tunable line from 680 nm to 1300 nm + 1040 nm fixed line for excitation of a range of fluorophores from blue to far red Variable dichroic-based spectral detection provides for simultaneous multi-channel acquisition 4 high QE non-descanned hybrid (HyD) detectors Heated animal platform and EZ-9000 anesthesia & nose cones for intravital imaging Objectives: 25X water immersion dipping objective (NA 0.95, w.d. 2.6 mm) 40X water coverslip corrected objective (NA 1.1, w.d. 0.65 mm) Upright configuration Scientifica stage and incubation with CO2 control Best for intravital imaging of dynamic in vivo processes and 3D (and 4D) volumetric imaging of thick tissue slices and organoids.