High Pressure Freezer

Capabilities

• ✓Physical immobilization of all cell components by ultrarapid freezing has been shown to be superior to conventional chemical fixation at room temperature.
• ✓The Leica EM rapid transfer system (RTS) allows correlative LM/EM experiments (CLEM), taking a live specimen from a light microscope (e.g. confocal) to freezing in less than 5 seconds.
• ✓High pressure freezing may be used to prepare samples for cryoTEM, cryoSEM, for subsequent preparation of freeze-fracture replicas for TEM, and for freeze-substitution. It has also been successfully applied to non-biological materials such as latex emulsions and inks for cryoSEM examination.