Grid Plunger

The Leica EM GP can be used for the vitrification of thin samples (up to a few microns) like viruses, proteins and cellular components as well as adherent cells for cryo-TEM and cryo electron tomography. Samples in suspension are applied onto grids in a humidity-controlled environmental chamber and plunge freezen into liquid ethane after removing excess fluid by automatic blotting. This is the bare grid technique. This instrument is purchased with funding from a NIH S10 Shared Instrument award.