Casper: LifeCanvas SmartBatch+ Electrophoretic Tissue Clearing and Immunolabeling Location Room 1042. 4 Blackfan circle (HIM) (Before using Casper) Protect tissue with SHIELD Preserve tissue architecture and fluorescent proteins with polyfunctional epoxide crosslinkers Protocol Delipidate quickly using Stochastic Electrotransport Disperse surfactant micelles throughout sample with rotational electric field Protocol Immunolabel with eFlash/SWITCH using Stochastic Electrotransport Achieve uniform whole-organ labeling quickly by modulating antibody binding affinity - allow uniform antibody diffusion without surface target saturation Protocol Validated Antibodies (After using Casper) Match Refractive Index with EasyIndex (RI 1.52) Protocol Please use your PPMS account to order samples and/or buffers for your clearing needs. In PPMS home page, navigate to "Order a service or consumable" and choose the appropriate service. Sample runs need to be a minimum of 4 samples.
Capabilities
- ✓Room 1042. 4 Blackfan circle (HIM)
- ✓Delipidate quickly using Stochastic Electrotransport Disperse surfactant micelles throughout sample with rotational electric field Protocol
- ✓Immunolabel with eFlash/SWITCH using Stochastic ElectrotransportAchieve uniform whole-organ labeling quickly by modulating antibody binding affinity - allow uniform antibody diffusion without surface target saturation Protocol Validated Antibodies
- ✓Achieve uniform whole-organ labeling quickly by modulating antibody binding affinity - allow uniform antibody diffusion without surface target saturation Protocol Validated Antibodies
- ✓(After using Casper) Match Refractive Index with EasyIndex (RI 1.52) Protocol
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