Megalodon LifeCanvas MegaSPIM

Location Room 0301. Veritas Science Center (Former NRB) Imaging Modality Lightsheet microscopy Volumetric imaging of large, optically clear samples Enables throughput imaging of multiple samples with different settings. Multi-immersion (water, wide range of clearing solutions, both organic based and water-based). Hardware Custom hardware ASI motorized stage XY stage range = 200mm. 16TPI scan-optimized (x axis) Laser lines: 405nm - 200mW 488nm - 150mW 561nm - 150mW 639nm - 150mW Emission filters Chroma ET445/58m Chroma ET525/50m Chroma ET600/50m Chroma ET690/50m 16bit Hamamatsu ORCA Fusion sCMOS monochrome camera Objective Lenses All objectives are multi-immersion dipping objectives 1.8X NA 0.1 working distance = 25mm, sample imagable thickness = 12mm Pixel size = 3.6um FOV = 7.2mm 3.6X NA 0.2 working distance = 18mm, sample imagable thickness = 8mm Pixel size = 1.8um FOV = 3.6mm 9X NA 0.3 working distance = 25mm, sample imagable thickness = 8mm Pixel size = 0.72um FOV = 1.4mm 22X NA 0.7 working distance = 10mm, sample imagable thickness = 2mm Pixel size = 0.3um FOV = 0.6mm All objectives are multi-immersion dipping objectives