Available
Widefield Microscopes
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Commercial Rate
The Keck Center’s widefield microscope systems use fluorescent lamp excitation, filter cubes, and CCD monochrome and/or RGB-color cameras to produce fluorescent, brightfield, DIC, Phase Contrast, and/or RGB-color images. The motorized XY-stage on the Leica DMI6000 microscope allows for mosaic tiling of large tissue sections, both fluorescent and color-stained, and multi-point live imaging.  Widefield microscopes do not exclude out-of-focus light as scanning confocal microscopes do, so they are usually not suitable for imaging thick samples.
Capabilities
- ✓Leica DMI6000 inverted microscope
- ✓5X LWD, 10X LWD, 20X, 20X LWD and 40X LWD dry objectives
- ✓40X and 100X Oil objectives
- ✓63X water objective
- ✓BFP (blue), CFP (cyan), GFP (green), YFP (yellow), TexasRed (red), and CY5 (far red) filter cubes
- ✓Motorized filters, shutters, and Z-drive
- ✓Motorized stage for mosaic tiling and/or multi-point live-imaging
- ✓Phase Contrast optics (for use with long working distance (LWD) objectives)
- ✓Z-sectioning
- ✓Mosaic tiling
- ✓Multi-point acquisition for live imaging
- ✓Leica DFC365 FX CCD camera (fluorescence, brightfield, DIC, phase contrast)
- ✓Leica MC170HD Color camera (color – e.g., crystal violet, histological stains)
- ✓LASX software with Navigator
- ✓Nikon Eclipse E600 upright microscope
