JEOL JEM-1400 Flash TEM

The JEOL JEM-1400 Flash TEM is equipped with: Dual-Axis Fischione Model 2040 specimen holder, which allows for precise in situ specimen in-plane 360-degree rotation that is necessary for automated montaging and serial imaging. This specimen holder features an optimal tilt angle range in narrow gap (~ 5 mm) pole piece geometries, while maintaining microscope resolution. Thus, this holder can also be used for 3D tomography applications. SerialEM and IMOD Software, which enables automated image acquisition, registration, and alignment of single- or dual axis tilt series. SerialEM also employs a versatile montaging system that can be used to acquire a large-scale image montage of the entire grid across the TEM sample. The IMOD software suite is a set of post-acquisition programs for processing the 3D tomography tilt series as well as serial section data into 3D reconstructions.