Nikon Spinning Disk
Nikon Spinning Disk
The spinning disk confocal platform within the Nikon Center of Excellence is outfitted with an array of high-end tools to enable multi-dimensional high-speed acquisition of live cells for extended periods of time. The system incorporates a Yokogawa CSU-X1 spinning disk head, high-speed piezo [z] stage, live cell incubator, automated and encoded X/Y stage, a four-line high-power solid state laser launch, and a full complement of objectives. In addition, a dedicated illumination path introduces a dedicated high-power 405nm diode laser directed by a pair of high-speed galvanometer-driven mirrors to enable targeted photo -activation, -switching/conversion, -bleaching, as well as photodamage. Both the piezo [z] stage as well as the emission filter wheel can be “triggered” by the camera, which enables very high speed acquisition of multichannel z-stacks over time. Camera Photometrics Prime 95B sCMOS monochrome camera Imaging Modes: Spinning disk confocal High-speed channel switching (multi-channel) High-speed multi-plane (“Z-stack”) Multi-position (x/y) Tile scans High-speed time-lapse Targeted (ROI-based) photo –activation, -conversion, -bleaching (FRAP), -ablation Excitation Specs: Full set of visual cubes for blue, green, and red –emitting probes. Diode Laser Lines and Power (measured at fiber tip): 405nm, 15mw (DAPI) 488nm, 15mW (FITC, GFP) 561nm, 15mW (TRITC, Cy3, mCherry) 647nm, 15mW (far-red, Cy5) Photo-stimulation Laser 405nm, 100mW Emission Specs: Emission filters for spinning disk confocal applications: 455nm (+/- 25nm) 525nm (+/- 18nm) 605nm (+/- 35nm) 641nm (+/- 75nm) 700nm (+/- 37nm) Dual Emission: 525nm (+/- 25nm) and 646 (+/- 66nm) Objective Lenses: Plan Fluor 4x 0.13 NA WD 12.2mm Plan Apo Lambda 10x 0.45 NA WD 4.00mm Plan Apo Lambda 20x 0.75 NA WD 1.00mm Plan Fluor 40x Oil DIC H N2 1.30 NA WD 0.20mm Plan Apo Lambda Oil 60x 1.40 NA WD 0.13mm Apo TIRF Oil 100x 1.49 NA WD 0.12mm Charges: $35.00 / hour Note: To use the microscope you must have an active Account with iLab. Contact Oleg to arrange training. Location MCN T-2216 NIKON COE
Capabilities
- ✓Photometrics Prime 95B sCMOS monochrome camera
- ✓Spinning disk confocal
- ✓High-speed channel switching (multi-channel)
- ✓High-speed multi-plane (“Z-stack”)
- ✓Multi-position (x/y)
- ✓High-speed time-lapse
- ✓Targeted (ROI-based) photo –activation, -conversion, -bleaching (FRAP), -ablation
- ✓Full set of visual cubes for blue, green, and red –emitting probes.
- ✓Diode Laser Lines and Power (measured at fiber tip): 405nm, 15mw (DAPI) 488nm, 15mW (FITC, GFP) 561nm, 15mW (TRITC, Cy3, mCherry) 647nm, 15mW (far-red, Cy5)
- ✓405nm, 15mw (DAPI)
- ✓488nm, 15mW (FITC, GFP)
- ✓561nm, 15mW (TRITC, Cy3, mCherry)
- ✓647nm, 15mW (far-red, Cy5)
- ✓Photo-stimulation Laser 405nm, 100mW
- ✓405nm, 100mW
Specifications
| SERVICE | *RATE/UNIT |
| LSM 510 Confocals, scope time | $46.00 / hour |
| Confocal, scope time scope time | $49.75 / hour |
| Widefield, scope time | $46.00 / hour |
| TEM, scope time | $113.00 / hour |
| Zeiss Crossbeam, scope time | $61.99 / hour |
| Nikon Spinning Disk, scope time | $35.00 / hour |
| Nikon SoRa, scope time | $35.00 / hour |
| Nikon SIM Super Resolution, scope time | $35.00 / hour |
| Nikon STORM Super Resolution, scope time | $35.00 / hour |
| Nikon TIRF, Multi-Excitation | $35.00 / hour |
| Zeiss Z.1 Lightsheet | $35.00 / hour |
| Embed (Spurr's) | $58.75 / capsule |
| Embed Durcupan | $58.75 / capsule |
| Embed SBEM | $113.00 / capsule |
| Freeze Substitution | $113.00 / capsule |
| High Pressure Freezer | $35.00 / capsule |
| Thick section | $59.74 / capsule |
| Thin section | $116.49 / capsule |
| Negative stain, material only | $6.00 / hour |
| ESEM prep, material only | $10.00 / hour |
| Sputter Coater | $10.00 / hour |
| CPD | $10.00 / hour |
| Imaging for hire, training, protracted help | $67.00 / hour |
| Analysis Workstations | $7.00 / hour |
Similar Instruments
