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Hammerhead
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The Zeiss LS7 lightsheet micrsoscoscope was funded through a NIH-S10 Instrumentation grant. NIH requires us to report every NIH grant/project that benefits from using this instrument. Additionally, NIH requires all researchers to acknowledge this grant in any publication using the instrument (Zeiss LS7). Please add the following grant to the funding sources/acknowledgments in any publication or grant submitted: Please use this grant number 1S10OD036362 Zeiss Lightsheet 7 with double side illumination For cleared and live samples (37°C incubator and CO2)
Capabilities
- ✓Room 0832. Veritas Science Center (Former NRB)
- ✓5x / NA-o.1 (for lower res illumination)
- ✓10x / NA - 0.2 (for higher res illumination)
- ✓5x/0.16 Plan Neofluar (for overview images of large samples)
- ✓10x/0.5 Plan Apochromat Water dipping (for live cell imaging of large samples or water-based-cleared samples)
- ✓20x/1.0 Plan Apochromat Water dipping (for high resolution live cell imaging)
- ✓20x/1.0 Plan Apochromat with correction collar (for clearing solutions with RI of 1.45)
- ✓20x/1.0 Plan Neofluar with correction collar (for clearing solutions with RI of 1.53)
- ✓Quad dichroic 405/488/561/638
- ✓emission filter (dual emission)DAPI (BP420-470) + GFP(BP505-545)DAPI (BP420-470) + CY3 (BP575-615)GFP (BP505-545) + mCherry, DRAQ5 (LP585)GFP (BP505-545) + DRAQ5 (LP660)Cy3 (BP575-615) + DRAQ5 (LP660)
- ✓DAPI (BP420-470) + GFP(BP505-545)
- ✓DAPI (BP420-470) + CY3 (BP575-615)
- ✓GFP (BP505-545) + mCherry, DRAQ5 (LP585)
- ✓GFP (BP505-545) + DRAQ5 (LP660)
- ✓Cy3 (BP575-615) + DRAQ5 (LP660)
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