Q-TOF instruments have a wide range of capabilities for characterization of small and large biomolecules. Ion mobility separation capabilities add an orthogonal separation based on gas-phase collisional cross-section (CCS). This instrument is primarily used in two modes, namely nanospray ionization for measurement of proteins under native conditions. IMS can be employed to measure changes in CCS in response to e.g. ligand binding or oligomerization state. Hydrogen-deuterium exchange is a powerful technique for measurement of protein-protein, protein-ligand interactions and protein dynamics. HDX automation enables experiments to be performed in an automated and highly reproducible fashion. Adding IMS enables complex mixtures of peptides to be separated in the HDX system based on HPLC elution time, gas phase structure, and peptide molecular weight.

Capabilities

✓Quadrupole time-of-flight mass spectrometry
✓Ion mobility separation (IMS)
✓Collisional cross-section measurement
✓Native protein analysis
✓Nanospray ionization
✓Hydrogen-deuterium exchange (HDX)
✓Protein-protein interactions
✓Protein-ligand interactions
✓Protein dynamics
✓HDX automation

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Specifications

model	Synapt G2Si
technique	Q-TOF with IMS
specialization	Native MS, HDX automation
ionization	Nanospray