Zeiss LightSheet.Z1 (Serenity)

-Selective plane illumination microscope (SPIM), excellent for imaging zebrafish, drosophila, and other small transparent organisms -Live cell incubation, heating and cooling -Objectives: 5x, 20x W dipping, 40x W -Excitation lasers: 405, 488, 561, and 635nm -Software platform: Zen 2012 LS Laser Details: Laser 405nm 50mW Laser 488nm 50mW Laser 561nm 50mW Laser 638nm 75mW Laser Blocking Filters and Filtersets: Filter Module LBF 405/488/561 Filter Module LBF 405/488/561/638 Filters for Two-Detector Modules Filter Module Lightsheet Z.1 – DAPI-GFP Filter Module LSFM – GFP-mCherry Filter Module Lightsheet Z.1 – GFP-DRAQ5 Filter Module Lightsheet Z.1 – CFP-YFP Zeiss website for their latest (Lightsheet 7) lightsheet microscopes Zeiss video on mounting a sample in an agarose-filled capillary Cancer Research Laboratory 449 Life Sciences Addition University of California, Berkeley Berkeley, CA 94720