Nikon Deconvolution Wide-Field Epifluorescence System

This system is good for: General fluorescence fixed and live cell imaging. Imaging of weakly fluorescent samples. Time-lapse live-cell imaging. This system is not good for: Very high Z axis resolution Fast bleaching dyes Function of Instrument This system uses a broadband mercury-halide light source and sets of high-throughput filters to excite fluorescent dyes and proteins. Overview At present we have 4 filter sets including: UV excitation and blue emission for DAPI, Hoechst, and Alexa 350 Blue excitation green emission for FITC, GFP, and Alexa 488 Yellow-green excitation red emission for Texas RED, Alexa 594, and mCherry Red excitation far red emission Alexa 647 (cy5) The system has motorized filter changer, nosepiece and focusing drive which allows for automated image acquisition. All imaging parameters are stored in the saved files for later analysis. The focus drive allows for stacks of images at different focal planes to be taken and that information is used to remove out of focus fluorescence and confocal like images to be acquired (deconvolution). The imaging device is a cooled sCMOS camera. This system is well suited for low intensity fluorescence samples.