Olympus FV-1000
Olympus FV-1000
This microscope is well suited for viewing polarized cells and tissue samples on sealed, inverted slides or in 35mm Matek dishes with a #1.5 coverglass. Imaging through a non-glass surface is discouraged. All slides MUST be sealed, clean and dry prior to placing on the stage. Imaging Modes: Laser scanning fluorescence and DIC (Nomarski) 3-D “Z-series” Time-series Simultaneous bleach/scan for PA-GFP and FRAP Fluorescence Filters: (excitation/emission) NearUV/Blue (405nm for UV dyes e.g, DAPI) Blue/Green (457,488,514nm for blue, green, yellow dyes, e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes) Green/Red (543nm for Cy-3, Texas Red, or rhodamine-like dyes) Red/Far-Red (633 nm for far-red dyes like Cy-5) Objective Lenses: 10x / 0.30 Plan Neofluar 20x / 0.80 SPlan-UApo 40x / 1.30 Plan-Neofluar OIL 60x / 1.45 Plan-Apochromat OIL 100x / 1.40 SPlan-UApo OIL Charges: $49.75 / hour Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar. Contact Sean to arrange training. Location: MRBIV 10434
Capabilities
- ✓Laser scanning fluorescence and DIC (Nomarski)
- ✓3-D “Z-series”
- ✓Time-series
- ✓Simultaneous bleach/scan for PA-GFP and FRAP
- ✓NearUV/Blue (405nm for UV dyes e.g, DAPI)
- ✓Blue/Green (457,488,514nm for blue, green, yellow dyes, e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes)
- ✓Green/Red (543nm for Cy-3, Texas Red, or rhodamine-like dyes)
- ✓Red/Far-Red (633 nm for far-red dyes like Cy-5)
- ✓10x / 0.30 Plan Neofluar
- ✓20x / 0.80 SPlan-UApo
- ✓40x / 1.30 Plan-Neofluar OIL
- ✓60x / 1.45 Plan-Apochromat OIL
- ✓100x / 1.40 SPlan-UApo OIL
- ✓MRBIV 10434
Specifications
| SERVICE | *RATE/UNIT |
| LSM 510 Confocals, scope time | $46.00 / hour |
| Confocal, scope time scope time | $49.75 / hour |
| Widefield, scope time | $46.00 / hour |
| TEM, scope time | $113.00 / hour |
| Zeiss Crossbeam, scope time | $61.99 / hour |
| Nikon Spinning Disk, scope time | $35.00 / hour |
| Nikon SoRa, scope time | $35.00 / hour |
| Nikon SIM Super Resolution, scope time | $35.00 / hour |
| Nikon STORM Super Resolution, scope time | $35.00 / hour |
| Nikon TIRF, Multi-Excitation | $35.00 / hour |
| Zeiss Z.1 Lightsheet | $35.00 / hour |
| Embed (Spurr's) | $58.75 / capsule |
| Embed Durcupan | $58.75 / capsule |
| Embed SBEM | $113.00 / capsule |
| Freeze Substitution | $113.00 / capsule |
| High Pressure Freezer | $35.00 / capsule |
| Thick section | $59.74 / capsule |
| Thin section | $116.49 / capsule |
| Negative stain, material only | $6.00 / hour |
| ESEM prep, material only | $10.00 / hour |
| Sputter Coater | $10.00 / hour |
| CPD | $10.00 / hour |
| Imaging for hire, training, protracted help | $67.00 / hour |
| Analysis Workstations | $7.00 / hour |
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