Zeiss Lattice Light Sheet Microscope

Light sheet fluorescence microscopy In light sheet imaging, samples are illuminated perpendicularly to the direction of observation using a thin sheet of light that is projected through the sample. Although the lateral resolution of a light sheet microscope is comparable to a widefield microscope, its key advantages are greatly reduced phototoxicity, rapid imaging, and the ability to image large samples from multiple directions for increased clarity and resolution. This technique has been masterfully adapted for use with cleared tissues, such as brain, kidney, and other organs measuring up to several millimetres in thickness System Name: Zeiss Lattice Light Sheet Microscope Objectives llumination Lens: 13.3x/0.4 (30 degrees) Detection Lens: 44.8x/1.0 (60 degrees) Camera pco.edge 4.2 Lasers 488 nm 561 nm 640 nm