Olympus IX81-DSU

Spinning disk confocal inverted microscope with motorized XY stage for fluorescence labeled samples. Available Tokai-Hit stage top incubator with active CO2 and temperature regulation and passive humidity, for imaging live cells in a 35mm dish. (The Tokia-Hit unit is CURRENTLY OFFLINE). Motorized XY stage movement for tile-mapping (AKA mosaic or stitching) and Z-drive for basic confocal Z-stack imaging. Mercury metal Halide white-light lamp for fluorescent excitation. Fluorescent filter sets: DAPI, GFP/FITC, TRITC, Texas Red, and CY5. Camera: Hamamatsu ORCA-AG (12 bit monochrome) Software: 3i SlideBook v4.2 (with Deconvolution module) on a Windows XP workstation. This software is also available on our separate Windows XP Analysis Workstation. The Olympus DSU (Disk Scanning Unit) offers leading-edge spinning disk confocal technology. Designed by Olympus, the DSU disk contains a pattern of slits that creates a virtual pinhole as the disk spins at 3,000 rpm. Designed to optimize the tradeoff between confocality and light throughput, the DSU uses an arc lamp illumination source for maximum excitation wavelength flexibility. Image formation is obtained from a CCD camera that allows full frame images to be acquired at up to 15 frames per second. The DSU is excellent for live-cell applications where speed of acquisition and minimal phototoxicity is paramount. Disk control is fully motorized allowing a computer to easily engage the disk into the lightpath and select wavelengths via t