NIIMBL Stellaris 8

The NIIMBL Stellaris 8 tauSTED/FLIM Confocal Microscope is a fully automated multimodal microscope that can be used for confocal, fluorescence lifetime imaging microscopy (FLIM) and super-resolution (STED). Applications: Confocal imaging of up to 5 colors simultaneously (far red dye with excitation and emission above 700nm can be imaged). FLIM can be used to separate multiple overlapping fluorophores or remove background signal. FLIM can also detect changes in the molecular environments of fluorophores and can be sensitive to multiple biomedical processes including disease progression and drug efficacy. FLIM can be used to follow fast molecular interactions via FLIM-FRET (Förster Resonance Energy Transfer) and FLIM biosensors can detect changes in metabolic state and microenvironment. 2D or 3D stimulated emission depletion (STED) is a super-resolution microscopy technique capable of improving spatial resolution by a factor of ~8 over standard confocal microscopy. Objectives: 20x/0.75 multi-immersion (water, glycerol, or oil), 40x/1.1 W MotCorr, 86x/1.2W MotCorr, 93x/1.3 Glyc MotCorr, 100x/1.4 Oil – a 10x/0.4 dry is also available upon request. MotCorr objectives can be used to compensate mismatch of refractive indexes and restore optimal imaging conditions particularly when imaging deeper into the samples. Laser lines: 405nm, white light laser (WLL) 440-790nm, 592nm STED, 775nm STED. The WLL is a tunable laser – one can select any laser lines between 440 and 790nm. Detectors: HyD S1, HyD X2, HyD S3, HyD X4, HyD R5. HyD detectors are 2 to 3 times more sensitive than regular photomultiplier detectors: Operating software: LAS X Location: APB 141U Contact Person: Dr. Sylvain Le Marchand