Nikon Epifluorescence Microscope w/ TIRF

TIRF microscopy is a specialized technique that allows for imaging of fluorescent molecules located within 200 nm of the coverslip. It accomplishes this by the generation of a fluorescent evanescent wave that decays exponentially as it projects upward from the surface of the coverslip. In doing so, only fluorophores located 100-200 nm from the coverslip are excited, resulting in very high signal-to-noise imaging of the basolateral surface of cells. This technique is especially useful for observing membrane associated processes such as ligand binding, cell adhesion, and vesicle trafficking from the cell surface. System Name: Nikon Epifluorescence Microscope w/ TIRF Objective 60x/1.49 (O) Camera Hamamatsu ORCA-Fusion BT Lasers 491 nm 561 nm