Confocal microscopes

The Nikon Super-Resolution Spinning Disk (SR-SD) is based on a Nikon Ti2 microscope equipped with a motorized XY stage, Piezo-actuated Z-stage, 4x, 10x, 20x, 40x, 60x and 100x lenses, a Yokogawa CSUX1 Spinning Disk head, GATACA Live-SR unit, Andor Zyla 4.2 sCMOS camera (2048x2048 pixels), and an LCI stage-top incubator for live-cell imaging. Fluorescence excitation is provided by 405/488/561 and 638nm lasers, with emission filters for DAPI+Far-Red/GFP/Texas Red/Far-Red and a 650nm longpass. The system is operated using µManager software. The Cicero spinning disk is a high-speed lenseless spinning disk system allowing rapid confocal imaging of living samples. The system is based on a Zeiss Axio Observer microscope equipped with motorized XYZ stage, 5x, 20x, 40x, 60x and 100x Plan-Apo lenses, a Hamamatsu Flash 4.0 LT camera (2048x2048 pixels) and an environmental chamber for long-term live imaging. Fluorescence excitation is provided by the 89North LDI-5 laser launch, with lines at 405/488/555/640nm, with emission filters for DAPI, Alexafluor488, Alexafluor 568 and a longpass far-red filter. The system is operated using Quorum's Volocity software or µManager. The Nikon Laser scanning C2 system is a fully motorized inverted microscope, equipped with  four lasers (405, 488, 561, 640 nm), for confocal imaging, bright 20x, 40x, 60x and 100x objective lenses; DIC optics, a motorized stage and a Nikon Perfect Focus autofocus system. The system is operated using NIS-Elements software. The Fluoview FV10i (or "confocal-in-a-box") is a self-contained confocal laser scanning microscope. It is outfitted with four laser diodes (405nm, 473nm, 635nm, 559nm) and uses a photomultiplier-based spectral system for detection. It has a 10× air and a 60× oil immersion objectives lens with the possibility to zoom up to 10 times to provide highly resolved phase contrast and fluorescent confocal images (up to 0.027 μm/pixel). The FV10i is equipped with an automatic detection of interface b