Available
Total Internal Reflection Fluorescence (TIRF) microscope for looking within ~100 nm of the microscope slide. Specifically well suited for endocytosis, exocytosis, adhesion and membrane protein/lipid studies. Fluorescence Emission Filters (TIRF) - separate widefield cubes are also avaliable. Detectors: (sCMOS are in place but can be switched for EM-CCD cameras depending on the application)
Capabilities
- ✓Total Internal Reflection Fluorescence (TIRF)
- ✓Variable pinhole Spinning Disk Confocal
- ✓Rapid simultaneous two colour imaging on EMCCD
- ✓Environmental Control for live cell imaging
- ✓Halogen Lamp for Transmitted Light
- ✓Wide Field Microscopy - X-Cite 120 LED (370-700nm), 100W
- ✓Diode Laser - 488nm - 100 mW - with FLCRtm control system
- ✓Diode Laser - 561nm - 100 mW
- ✓Diode Laser - 642 nm - 110 mW
- ✓10X N-PLAN, NA=0.25
- ✓20X HC PLAN APOCHROMAT, NA=0.70,
- ✓63X HCX PLAN APOCHROMAT, NA=1.47, OIL, Corr, TIRF
- ✓EGFP (ET 525/50nm)
- ✓mCherry (ET 620/60nm)
- ✓EGFP/mCherry Dual Cube
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