Leica TCP SP8 with diode lasers and light-sheet

The Leica TCS SP8 system possesses fixed diode laser lines (405, 488, 552, 638 nm) that can be utilized for conventional confocal microscopy or digital light-sheet applications. Illumination with the light sheet allows rapid acquisition of multiple images (>100 frames/second) to permit 3-D rendering and results in low phototoxicity. Imaging can be performed for entire organs (e.g., Arabidopsis roots) or organisms (e.g., Drosophila embryos, developing zebra fish). Extended time-lapse acquisition can be used to monitor physiological or developmental changes over time. Download a PDF describing the basics of light-sheet microscopy and providing protocols for sample preparation. The following objectives are available for use with the confocal and light-sheet systems: The following objectives are specifically for use with the light-sheet system: