Zeiss LSM980

The Zeiss LSM980 is an upright confocal microscope (i.e. objectives are above the sample) that can be used for confocal, spectral, and multiphoton imaging. Applications: Confocal imaging. Spectral imaging with linear unmixing. It can be used to remove background autofluorescence or for multicolor imaging when spectral overlaps between colors cannot be separated with standard confocal microscopy. Multiphoton excitation microscopy. It can be used to image up to 10x deeper than confocal microscopy. Two non-descanned PMT detectors can be used for imaging multiphoton fluorescence or second harmonic generation (SHG) in the forward and reverse directions. SHG is now widely used to image collagen in many different applications, and is becoming useful for imaging myosin and some polysaccharides. Confocal reflection microscopy. It can be used to image collagen fibers and surface microtopography. FRET, FRAP, FLIP. Fluorescence Correlation Spectroscopy imaging. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions. Tile scanning to image a large field at high resolution. Objectives: 10x/0.3, 20x/0.75, 40x/1.2 W, 40x/1.4 Oil – more objectives are available as add-ons. Laser lines: 405nm, 488nm, 561nm, 639nm, 690-1040nm. Detectors: a 32-channel ultra-sensitive spectral GaAsP PMT (Gallium Arsenide Phosphide PhotoMultiplier Tube) detector (i.e. ChS in Zen) which is ideal for spectral imaging, two GaAsP PMT detectors (Ch1 & Ch2), one transmitted PMT (T-PMT) for contrast imaging (Bright Field, Oblique illumination, Differential Interference Contrast), and two non-descanned detectors (one GaAsP and one multi-alkali) for multiphoton imaging. Operating software: Zen 3.5 Location: CBBI 244C Contact person: Dr. Sylvain Le Marchand