Leica Stellaris 8 FALCON w/STED

Stimulated Emission Depletion (STED) STED microscopy overcomes the diffraction limit by overlapping the primary excitation laser with a secondary, donut-shaped, high-power laser beam. Irradiation by the secondary laser forces molecules in the outer region of the primary excitation area to return to the ground state, while leaving fluorophores in the centre of the excitation focus to form the high resolution image. This application can achieve resolutions up to 50 nm laterally, and up to 120 nm axially, and is best suited for 2D or 3D imaging of thin samples (<50 um) that have excellent fluorescence labeling. System Name: Leica Stellaris 8 FALCON w/STED Objectives 93x/1.3 STED (motCORR) 100x/1.4 STED Lasers 405 nm white light laser (pulsed, 440-790 nm) 775 nm (pulsed, STED) Other TauSTED