Zeiss Alyra PS1 Andor iXon DU897

Localization Microscopy (STORM/PALM) Stochastic optical reconstruction microscopy (STORM) and photoactivated localization microscopy (PALM) function by stochastically cycling fluorophores between ‘on’ and ‘off’ states in a controlled manner, followed by precise localization of the centre of each fluorescent molecule to within tens of nanometers. By processing thousands of images, the molecular coordinates of all labeled molecules are obtained with a resolution ten times better than traditional diffraction limited microscopes, i.e. approximately 20 nm (XY), 50 nm (Z). This technique is best suited for imaging the stoichiometric architecture of proteins within defined complexes, and is largely limited to fixed, cellular samples. System Name: Zeiss Elyra PS1 Objectives 63x/1.4 PlanApo 100x/1.46 alpha PlanApo Cameras Andor iXon DU897 (PALM) Lasers 405 nm (150 mW) 488 nm (200 mW) 561 nm (200 mW) 640 nm (150 mW) Other 3D: double-helix PSF