Laser scanning confocal microscope for optical sectioning, z-stacking and tile scanning. Suitable for fixed and live cells, tissue sections and organoids up to ~100 μm thickness. Unique advantages of this microscope include the Airyscan 32-hexagonal array detector enabling super-resolution. Each member of the array mimics a pinhole with an aperture of 0.2 Airy Unit, together collecting the entire point spread function, maximizing resolution and sensitivity. Modes of operation: Conventional, point-by-point laser scanning Airyscan array super resolution imaging UVA laser microirradiation 96 well plate scanning Photomanipulations (FRET/FRAP) Spatial resolution ~140 nm XY and ~400 nm Z Excitation lasers: 405, 488, 561, 594 and 633 nm 355 nm OPSL laser for DNA damage repair studies 32-channel GaAsP spectral detector (Airyscan) plus 2 PMTs and transmitted light PMT DIC/Nomarski optics to enhance transmitted light contrast XL incubator chamber and CO2 control for live cell and tissue imaging Best for high-resolution imaging of cells and medium thickness fluorescent specimens across the complete spectral range, and UVA laser microirradiations to assess live cell response to DNA damage.
