Nikon PRIMO
The Nikon PRIMO is a Nikon Ti-2 microscope with widefield fluorescence and an Alveole PRIMO attachment. The PRIMO is a micropatterning, hydrogel polymerization and microfabrication device that allows you to create custom made in vitro cellular microenvironments. It uses a digital micromirror device (DMD) technology that illuminates a pattern with UV light at 375nm.The Nikon Ti-2 is equipped with the D-LEDI fluorescent LED light source and Prime BSI Express Scientific CMOS (sCMOS) camera. The PRIMO also has a white LED light source and Nikon DS-Ri-2 color camera for bright field imaging of histology stained samples. The microscope offers a 22mm FOV image. Camera: Prime BSI Express Scientific CMOS (sCMOS) camera Nikon DS-Ri2 color camera Imaging Modes: PRIMO – micropatterning, hydrogel polymerization, and microfabrication Chromogenic microscopy (DAB, H&E, etc) Conventional fluorescence microscope Excitation Specs: Nikon D-LEDI LED light engine: 385 nm (DAPI) 475 nm (FITC. GFP) 550 nm (TRITC, Cy3) 621 nm (far red, Cy5) Objective Lenses: 4x Plan Fluor NA 0.13 WD 17.1mm 10x Plan Fluor DIC N1 NA 0.3 WD 16mm 20x Plan Apo λD OFN25 DIC N2 NA 0.8 WD 800um 20x S Plan Fluor ELWD DIC N1 NA 0.45 WD 8.2mm (for PRIMO) 40x Plan Fluor Oil DIC H N2 NA 1.3 WD 240um Charges: $35.00 / hour Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar.Contact Tegy, or Oleg to arrange training. Location: MCN T-2216
Capabilities
- ✓Prime BSI Express Scientific CMOS (sCMOS) camera
- ✓Nikon DS-Ri2 color camera
- ✓PRIMO – micropatterning, hydrogel polymerization, and microfabrication
- ✓Chromogenic microscopy (DAB, H&E, etc)
- ✓Conventional fluorescence microscope
- ✓Nikon D-LEDI LED light engine: 385 nm (DAPI) 475 nm (FITC. GFP) 550 nm (TRITC, Cy3) 621 nm (far red, Cy5)
- ✓385 nm (DAPI)
- ✓475 nm (FITC. GFP)
- ✓550 nm (TRITC, Cy3)
- ✓621 nm (far red, Cy5)
- ✓4x Plan Fluor NA 0.13 WD 17.1mm
- ✓10x Plan Fluor DIC N1 NA 0.3 WD 16mm
- ✓20x Plan Apo λD OFN25 DIC N2 NA 0.8 WD 800um
- ✓20x S Plan Fluor ELWD DIC N1 NA 0.45 WD 8.2mm (for PRIMO)
- ✓40x Plan Fluor Oil DIC H N2 NA 1.3 WD 240um
Specifications
| SERVICE | *RATE/UNIT |
| LSM 510 Confocals, scope time | $46.00 / hour |
| Confocal, scope time scope time | $49.75 / hour |
| Widefield, scope time | $46.00 / hour |
| TEM, scope time | $113.00 / hour |
| Zeiss Crossbeam, scope time | $61.99 / hour |
| Nikon Spinning Disk, scope time | $35.00 / hour |
| Nikon SoRa, scope time | $35.00 / hour |
| Nikon SIM Super Resolution, scope time | $35.00 / hour |
| Nikon STORM Super Resolution, scope time | $35.00 / hour |
| Nikon TIRF, Multi-Excitation | $35.00 / hour |
| Zeiss Z.1 Lightsheet | $35.00 / hour |
| Embed (Spurr's) | $58.75 / capsule |
| Embed Durcupan | $58.75 / capsule |
| Embed SBEM | $113.00 / capsule |
| Freeze Substitution | $113.00 / capsule |
| High Pressure Freezer | $35.00 / capsule |
| Thick section | $59.74 / capsule |
| Thin section | $116.49 / capsule |
| Negative stain, material only | $6.00 / hour |
| ESEM prep, material only | $10.00 / hour |
| Sputter Coater | $10.00 / hour |
| CPD | $10.00 / hour |
| Imaging for hire, training, protracted help | $67.00 / hour |
| Analysis Workstations | $7.00 / hour |
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