Leica STELLARIS 8 tauSTED Super Resolution Microscope

This system equipped with an inverted microscope, coupled to a confocal, FLIM (FALCON), and tauSTED microscopy system. The Leica STELLARIS 8 tauSTED (Stimulated Emission Depletion) is a fully integrated system with FALCON (FAst Lifetime CONtrast) imaging, providing fast, intuitive access to structural details inside the sample, far beyond the diffraction limit. The tauSTED microscope is based on a Leica STELALRIS 8 spectral confocal scan head. The system has unique features that allow for unsurpassed flexibility and sensitivity in confocal, lifetime (FLIM), and STED imaging microscopy. The lateral resolution of Leica tauSTED is about 20-30 nm for fixed specimen and <70 nm for live specimens. The Z resolution is about 200nm. tauSTED Depletion lines - The tauSTED configuration has three STED depletion laser lines: 592 nm, 660 nm, and 775 nm to cover the complete visible spectrum for the far-red fluorophores. The notch filter set allows to block the excitation and depletion light in order to improve the efficiency of the fluorescence emission signal. As mentioned in the confocal below, the WLL laser lines (440-790 nm) are required to excite labeled fluorophores to acquire tauSTED images. Leica STELLARIS 8 confocal system equipped with pulsed white light laser (WLL; repetition rate 78MHz) and tunable excitation wavelengths from 440 nm to 790 nm [Laser power per line: > 0.9 mW 440-485 nm, > 1.8 mW 485-790 nm; if more power is required more lines (up to 8 lines) can be used simultaneously]. This WLL will be used in both, confocal and tauSTED mode. In addition, the system will include a 405 nm excitation laser line in a much-needed spectral range not covered by the WLL. The enhanced detection efficiency and the optimal match of excitation and detection enable long time live cell imaging. The combination of Field-of-View (FOV) or Tandem scanner with rolling average and LIGHTNING delivers superb image quality at full speed and high resolution (120-140 nm) with better SN