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CIAN-SP8 – Confocal Laser Scanning
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Leica SP8 point-scanning confocal system, on a Leica DMI6000B inverted microscope, fully motorized, with Leica “SuperZ” Galvo stage, four spectral fluorescent light detectors (three PMT, one HyD high sensitivity detector), one transmitted light detector (PMT) Lasers, no fluorescence emission filters (all spectral detectors): Laser modulation by AOTF (acusto-optic tunable filters), or DMDO (direct modulation of laser intensity)
Specifications
| 1 | 10x/0.4 dry HC PL APO 1.x/0.40 CS |
| 2 | 20x/0.7 dry HC PL APO 20x/0.70 CS |
| 3 | 40x/0.85 dry HCX PL APO 40X/0.85 CORR CS,0.11- |
| 4 | 40x/1.1 water HC PL APO 40x/1.10 W CORR CS2 |
| 5 | 40x/1.3 oil HC PL APO 40x/1.30 Oil CS2 |
| 6 | 63x/1.4 oil HC PL APO 63x/1.40 OIL CS2 |
| Position | Objective |
| Lasers (all diode) | Typical fluorophore |
| 405nm, 50mW, DMOD | DAPI |
| 448nm, 40mW, AOTF | CFP |
| 488nm, 20mW, AOTF | AF488, GFP, YFP |
| 552nm, 20mW, AOTF | TRITC, Cy3 Texas Red, RFP |
| 638nm, 30mW, AOTF | AF633, Cy5, FarRedFP |
| Filter set | Excitation filter |
| I3 | BP450-490 |
| N2.1 | BP515-560 |
| A | BP340-380 |
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