Leica Thunder Imager

The Leica Thunder Imager is an upright microscope for fluorescence, transmitted light, and true color imaging . With a stage capable of holding up to 8 slides, this instrument is optimized for high throughput imaging of cells and tissue sections. Fluorescent images can be processed with Leica’s computational clearing and deconvolution to remove out of focus blur, enhance contrast, and sharpen details. The software Navigator function can be used for rapidly generating sample overviews for subsequent selection of areas of interest. The instrument is located in the Biology and Physics Building (BPB), room G05D. Specifications: Microscope: Upright Stand Leica DM6 B Motorized x-y stage with 8 slide holder Fluorescent Illuminator: LED3 solid state white light source Fluorescent Filter Cubes DAPI: Ex 405/60, Em 470/40 GFP: Ex 470/40, Em 525/50 Y3: Ex 545/25, Em 605/70 Y5: Ex 620/60, Em 700/75 Cameras: Leica K5 sCMOS monochrome camera, 2048 x 2048 pixels (for fluorescence imaging) Leica DMC5400 CMOS color camera (for histology dyes) Objective Lenses: 2.5x/0.07 Plan 5x/0.15 Plan Fluotar 10x/0.45 Plan Apo 20x/0.80 Plan Apo, DIC 40x/1.30 Plan Fluotar Oil, DIC 63x/1.40 plan Apo Oil, DIC 63x/1.30 Plan Apo Glycerol Software: LAS X for image acquisition, processing, and quantification. Leica Thunder software module includes Instant Computation Clearing (ICC) Small Volume Computation Clearing (SVCC) and Large Volume Computational Clearing (LVCC)