Nikon AXR Confocal + TIRF

The Nikon AXR is a point scanning confocal built around the inverted Ti2E microscope. The large 25 mm field of view and resonant scanner are optimized for rapid imaging of samples. The DUX-VB confocal detector features 4 sensitive GaAsP detectors that can be flexibly configured for a range of dyes via spectral detection. 6 laser lines provides a range of excitation options for a variety of fluorescent dyes. In addition to confocal microscopy, the system has an iLAS2 illumination module for TIRF imaging. This device can perform simultaneous TIRF imaging and photostimulation. TIRF, widefield, and transmitted light images are collected on a Photometrics Prime 95B sCMOS camera. The instrument is located in the Biology and Physics Building (BPB), room G05D Specifications Microscope: Inverted microscope Nikon Ti2E Motorized x/y stage PerfectFocus optical z drift correction MCL Nano-Z200 piezo z stage Laser Unit for Confocal: LUA-S6 405/445/488/514/561/640 Laser Unit for TIRF: LUNF XL 405/488/561/640 TIRF/Widefield Camera Photometrics Prime95B Objective Lenses: 10X PLAN APOCHROMAT LAMBDA D, DIC 20X PLAN APOCHROMAT LAMBDA D, DIC 40X/1.30 PLAN FLUOR, Oil, DIC 60X/1.40 PLAN APOCHROMAT LAMBDA D, Oil, DIC 60X/1.20 PLAN APO, WI, DIC 100X/1.49 APO TIRF, Oil, DIC Software: NIS-Elements C Confocal Package, JOBS for Scanning Plates and Creating Custom Acquisition and Analysis Routines, NIS-Elements 2D/3D, Deconvolution Suite, NIS.ai Artificial Intelligence Module, General Analysis 2 and 3