Andor Dragonfly

The Dragonfly microscope is a fully automated microscope that can be used for confocal and total internal reflection microscopy (TIRF). Applications: Spinning disk confocal imaging. In contrast to point scanning confocals, such as the LSM880 and CD7 that serially acquire one focal point at a time to reconstitute a 2D image, the Dragonfly acquires confocal images by simultaneously acquiring thousands of spots from the focal plane. This setup enables the Dragonfly to provide several important advantages over point-scanning confocals: Faster speed (up to ~200 frames per second), which is ideal for fast 2D or 3D time-series or tile scanning. Gentler imaging – photobleaching and phototoxicity are greatly reduced, which is better for live cell imaging. The high sensitivity of the camera is well suited for dim specimens. TIRF microscopy. a technique allowing the confined excitation of fluorophores within ~100nm above the coverslip. Objectives: 1.25x/0.04, 10x/0.45, 20x/0.8, 40x/1.1 W, 63x/1.47 Oil. Laser lines: 405nm, 488nm, 561nm, 637nm. Detectors: Andor Sona sCMOS Operating software: Fusion Location: Building X, 032B Contact Persons: Dr. Sylvain Le Marchand (confocal), Tim Chaya (TIRF)