Zeiss LSM880

The LSM880 microscope is a fully automated multimodal microscope that can be used for confocal, spectral, and multiphoton excitation imaging. The Airyscan detector enables super-resolution and high speed imaging. The LSM880 is fully equipped for live-cell imaging. It has an environmental enclosure (temperature, humidity, and CO2 control) and z-drift correction to maintain samples at the focal plane over time. Applications: Confocal imaging. Spectral imaging with linear unmixing. It can be used to remove background autofluorescence or for multicolor imaging when spectral overlaps between colors cannot be separated with standard confocal microscopy. Multiphoton excitation microscopy. It can be used to image up to 10x deeper than confocal microscopy. Super-resolution imaging. The Airyscan can provide a 2-fold increase in resolution compared to conventional confocal microscopy. High-speed imaging. The Airyscan detector can be used to image 4 times faster than the confocal mode (up to 20fps at 512x512). The LSM880 is also equipped with a piezo-electric stage for high-speed acquisition of z-stacks. Confocal reflection microscopy. It can be used to image collagen fibers and surface microtopography. FRET, FRAP, FLIP. Fluorescence Correlation Spectroscopy imaging. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions. Tile scanning to image a large field at high resolution. Live cell imaging thanks to the environmental enclosure. Objectives: 10x/0.3, 20x/0.8, 40x/1.2 W, 40x/1.3 Oil, 63x/1.4 Oil – more objectives are available as add-ons. Laser lines: 405nm, 458nm, 488nm, 514nm, 561nm, 633nm, 680-1080nm. Detectors: a 32-channel ultra-sensitive spectral GaAsP PMT (Gallium Arsenide Phosphide PhotoMultiplier Tube) detector (i.e. ChS in Zen) which is ideal for spectral imaging, two multi-alkali PMT detectors (Ch1 & Ch2), one transmitted PMT (T-PMT) for contrast imaging (Bright Field, Differential Interference Contrast, Phase), and one GaAsP Airyscan detector for super-resolution and fast imaging. Operating software: Zen 2.3 SP1 Location: APB 141S Contact person: Dr. Sylvain Le Marchand