BD Biosciences LSRII Cell Analyzer

BD Biosciences' LSRII cell analyzer was recently upgraded with funds from the Oklahoma Center for Adult Stem Cell Research (OCASCR). It now utilizes four lasers (407 nm, 488 nm, 561 nm and 633 nm air-cooled lasers) and precise optics to detect up to 16 independent signals. The 488 nm laser can be used to detect common fluorochromes including GFP, FITC and PerCP. The 561 nm laser is great for exciting PE and PE tandem dyes. The 633 nm laser is used to detect APC, APC-Cy7, APC-Cy5.5 and Cy5. These fluorochromes are most commonly used by our investigators who study hematopoietic stem cells and committed progenitors or B cell subsets from tonsils. Finally, the 407 nm laser is appropriate for FRET experiments, exciting Quantum Dots and for using Hoechst 33342 to study side population cells.