Sauron

Location Room 1023. 4 Blackfan circle (HIM) Imaging Modality Widefield TIRF/Ring-TIRF HiLo illumination Focal point ablation Live cell imaging Hardware Ti2E Motorized Inverted Microscope LUN-F Laser Launch with two fiber outputs. Fiber 1: 488nm (60mW),561nm (50mW) and 640nm (40mW). Fiber 2: 405 (50mW), iLAS2 TIRF/Photostimulation illuminator Back aperture observation camera Filters C-TIRF Ultra High Signal-to-Noise 405/488/561/638nm Quad TIRF Filter Set, Quad 405/488/561/638nm Laser Cleanup, HC TIRF 405/488/561/638nm Quad Dichroic Mirror in Metal Cube, HC Quad Barrier Filter and three Supplementary BP 525/50nm, 600/50nm and 700/75nm HC Laser Block Barrier Filters 25mm for Emission Filter Wheel. (6 filters total) C-FL DAPI SOLA Hard Coat, High Signal-to-Noise, Zero Shift Filter Set, Excitation: 395/25nm (382.5-407.5nm), Emission: 460/50nm (435-485nm), Dichroic Mirror: 425nm C-FL GFP Hard Coat, High Signal-to-Noise, Zero Shift Filter Set, Excitation: 470/40nm (450-490nm), Emission: 525/50nm (500-550nm), Dichroic Mirror: 495nm C-FL DS Red Hard Coat, High Signal-to-Noise, Zero Shift Filter Set, Excitation: 545/30nm (530-560nm), Emission: 620/60nm (590-630nm), Dichroic Mirror: 570nm C-FL CY5 Hard Coat, High Signal-to-Noise, Zero Shift Filter Set, Excitation: 620/60nm (590-650nm), Emission: 700/75nm (663-738nm), Dichroic Mirror: 660nm KINETIX 22 sCMOS camera Enviromental control Nikon Elements Acquisition Software AR Objective Lenses Apo TIRF 100x/1.49 DIC N2