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Nikon Super Resolution Spinning Disk Confocal (NEW!)
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The Nikon Super Resolution Spinning Disk Confocal (SoRa) is a fully-loaded live cell imaging platform with dual disks (SoRa 50 μm) for super resolution and standard confocal imaging, Hamamatsu Quest 2 qCMOS & Andor iXon 888 EMCCD and, full incubation. The system is capable of up to 120nm resolution with the SoRa disk and 10s of nm with SMLM. DNA-paint and some dyes are possible for SMLM, but please speak to the imaging team prior to your experiment. Hamamatsu ORCA-Quest2 4096 x 2304 pixels, 4.6 µm pixel size Andor iXon Ultra 888 EMCCD, 1024x1024pixels, 13um pixel
Capabilities
- ✓Nikon Ti2E Inverted Microscope
- ✓Yokogawa CSU-W1 dual-disk spinning disk with 25 μm & 50 μm pinholes
- ✓Dual camera
- ✓Motorized stage + piezo drive
- ✓Perfect Focus System
- ✓Full incubation with OKO Labs chamber
- ✓Plan-Apochromat 4x/0.2 WD=20mm
- ✓Plan-Apochromat 10x/0.45 WD=4mm
- ✓Plan-Apochromat 25x/1.05 Silicon WD=0.55mm
- ✓Apochromat Lambda S LWD 40x/1.15 Water WD=0.59mm
- ✓Plan-Apochromat 60x/1.42 Oil WD=0.15mm
- ✓Apochromat TIRF 60x/1.49 Oil WD=0.12
- ✓405/488/568/647
- ✓405/488/568
Specifications
| Filter | Common Fluorophores |
| 447/60 nm | DAPI/ Alexa 405 |
| 520/35 nm | GFP/ Alexa 488 |
| 605/52 nm | TRITC/ Alexa 555/ Alexa 568 |
| 705/72 nm | Cy5/ Alexa 647 |
| Quad | DAPI/FITC/TRITC/CY5 |
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