FLIMer
Location Room 0084. Veritas Science Center (Former NRB) Imaging Modality Single point laser scanning confocal FLIM (fluorescence lifetime imaging - white light laser with pulse picker for capturing longer lifetimes) Multiplexing (>6 color imaging) Fixed and Live samples Transmitted light Live cell imaging (environmental enclosure with temperature control) Hardware Leica Inverted DMi8 Leica Stellaris 8 Falcon Motorized stage (ITK) Laser lines: 405 diode, WLL (440-790 tunable; pulse picker) Confocal detectors: 2 HydS, 2 HyDX, 1 HyDR, all spectral Full environmental enclosure for live imaging LasX acquisition software Objectives Plan Apo 10x/0.40 Air Fluotar 16x/0.6 multi-immersion. Correction collar from water to RI 1.57 Plan Apo 20x/0.75 Air Plan Apo 40x/1.25 Glycerol Plan Apo 63/1.40 Oil
Capabilities
- ✓Room 0084. Veritas Science Center (Former NRB)
- ✓Single point laser scanning confocal
- ✓FLIM (fluorescence lifetime imaging - white light laser with pulse picker for capturing longer lifetimes)
- ✓Multiplexing (>6 color imaging)
- ✓Fixed and Live samples
- ✓Transmitted light
- ✓Live cell imaging (environmental enclosure with temperature control)
- ✓Leica Inverted DMi8
- ✓Leica Stellaris 8 Falcon
- ✓Motorized stage (ITK)
- ✓Laser lines: 405 diode, WLL (440-790 tunable; pulse picker)
- ✓Confocal detectors: 2 HydS, 2 HyDX, 1 HyDR, all spectral
- ✓Full environmental enclosure for live imaging
- ✓LasX acquisition software
- ✓Plan Apo 10x/0.40 Air
