Agilent 2100 Bioanalyzer

The Core Facility staff offers analysis of nucleic acid samples (both RNA and DNA) using both the Agilent 2100 Bioanalyzer and Agilent (formerly AATI) Fragment Analyzer. This instrument provides an objective measure of nucleic acid quality that is more informative and more sensitive than traditional agarose gel results, without the use of toxic ethidium bromide and with reduced run times. Both systems combine gel-filled (micro)-capillaries, microfluidics and voltage induced separation with laser induced fluorescence detection of the analyzed nucleic acids. Neither instrument will provide information about chemical contaminants such as phenol, salts, proteins, ethanol etc. They provide limited information on the concentration of a sample and they are not recommended if an accurate concentration is required for a downstream application.