Super Resolution Systems

The Facility Line STED microscope from Abberior Instruments has four lasers for excitation (405, 485, 561, 640 nm) as well as two pulsed STED lasers (595 and 775 nm). Patented adaptive illumination methods enable live-cell imaging with drastically reduced photobleaching and phototoxicity. FLIM capabilities available through two channel TCSPC. STED super-resolution microscope on an inverted Olympus IX83 body Two pulsed STED lasers (595 and 775 nm) complement four excitation lasers (405, 485, 561, 640 nm) Okolab stage top incubator for CO2 and temperature control (Uno-T line) Multi-channel, z-stacks, time-lapse imaging, multi-point capture, tiling Z-drift compensation unit FLIM, FRAP, FLIP, FRET, photoactivation, colocalization Software options include: SymPhoTime 64--Picoquant data acquisition and analysis software Objectives 10X NA 0.4 WD 3.1mm UPLXAPO Air 20X NA 0.75 WD 0.6mm UPLSAPO20 Air 40X NA 1.4 WD 0.13mm UPLSAPO40XO Oil 60X NA 1.2 WD 0.28mm UPLSAPO60XW 60X NA 1.3 WD 0.3mm UPLSAPO60XS Sil 60X NA 1.42 WD 0.15mm UPLXAPO60XO Oil Excitation laser lines (nm) 405 485 561 640 405 - CW, 20 mW 485 - ~1mW pulsed 561 - ~200 µW pulsed 640 - ~1mW pulsed Pulsed STED lasers (nm) 595 775 595 - up to 400 mW 775 - >2750 mW @ 40 MHz, repetition rate 25-40 MHz Detectors Four ultra-sensitive single-photon-counting APDs with tunable detection bands between 400 and 800nm Stage IX3-SSU ultrasonic xy-scanning stage for IX83 Autofocus Custom design based on Olympus IX3-ZDC-12 z-drift compensation unit for steady confocal/STED imaging Stage top incubator Okolab, Uno-T line with temperature control up to 40°C