3i Cleared Tissue Light Sheet Microscope

AxL Cleared Tissue LightSheet (AxL CTLS) is a fully automated macro zoom microscope with high NA apochromatic objectives and dual-sided light sheet illumination for imaging whole organs to small animals. Custom-designed excitation objectives and patented axially swept light sheet microscopy (ASLM) produce an exceptionally thin, long and uniform lightsheet for large-scale high-resolution imaging. Using a back-thinned sCMOS camera, AxL CTLS images one cubic centimeter in less than a minute. Proprietary dual-sided light sheet illumination optics Compact 54 x 60cm footprint Seamlessly switch imaging modes without changing optics - Axially swept light sheet mode & Ultrafast 3D prescan mode 2304 x 2304 6.5µm pixels 95% quantum efficiency Rolling shutter mode Axial light sheet sweep length of 10mm for large field of view (FOV) 1.0x/0.25NA, WD 56mm 1.5x/0.37NA, WD 30mm 2.3x/0.57NA, WD 10mm Thin and uniform sheet across a 10mm FOV Adjustable FOV for faster imaging of smaller specimens Spherical aberration correction for high-refractive-index immersion media Flat excitation profile across 1mm to 10mm sheet width 0.14NA diffraction-limited light sheet Sample chambers in 4 different sizes Temperature controlled Chemically-inert sample holders Clearing solutions ranging from 1.33-1.56 RI Multi-specimen holder for up to 10 different samples 3i’s SlideBook software CTLS control module manages every step in cleared tissue imaging. An intuitive workflow guides users through the collection of 3D stacks, 3D data montaging, volume rendering and finally movie making with story-board support. SlideBook is GPU optimized and readily handles the creation and processing of 3D datasets at over 1TB, making them ready for analysis and rendering. SlideBook .SLD files can be accessed via any application supporting Bio-Formats OME, allowing seamless collaboration in any workflow. Select a color scheme from dozens of options Switch on-the-fly from dark to light themes Directory-based open