Zeiss Gaussian Lightsheet 7 (LS7) Microscope

This microscope is preferred for 3-D imaging of thick specimens such as biopsies or mouse whole organs (up to 20 mm) that are optically clarified using special tissue clearing procedures. Illumination orthogonal to detection optics generates a thin light sheet for selective planar illumination of the imaging plane, sparing the remainder of sample excitation thereby permitting rapid FOV camera-based imaging of the entire volume and minimizing sample bleaching. Modes of operation Dual sided lightsheet illumination Single sided lightsheet illumination 6 laser lines (405, 445, 488, 514, 561 and 638 nm) for rapid multispectral imaging from blue to far-red 5X and 10X illumination optics Detection optics include 5X and 20X water and clearing (mixed immersion) 2 high speed (57 fps at 1K x 1K) and high quantum efficiency (83% QE) PCO Edge 4.2 sCMOS cameras Best for rapid volumetric imaging of fixed and cleared whole organs, tissues and embryos.