Nikon Spinning Disk

The spinning disk confocal platform within the Nikon Center of Excellence is outfitted with an array of high-end tools to enable multi-dimensional high-speed acquisition of live cells for extended periods of time. The system incorporates a Yokogawa CSU-X1 spinning disk head, high-speed piezo [z] stage, live cell incubator, automated and encoded X/Y stage, a four-line high-power solid state laser launch, and a full complement of objectives. In addition, a dedicated illumination path introduces a dedicated high-power 405nm diode laser directed by a pair of high-speed galvanometer-driven mirrors to enable targeted photo -activation, -switching/conversion, -bleaching, as well as photodamage. Both the piezo [z] stage as well as the emission filter wheel can be “triggered” by the camera, which enables very high speed acquisition of multichannel z-stacks over time. Camera Photometrics Prime 95B sCMOS monochrome camera Imaging Modes: Spinning disk confocal High-speed channel switching (multi-channel) High-speed multi-plane (“Z-stack”) Multi-position (x/y) Tile scans High-speed time-lapse Targeted (ROI-based) photo –activation, -conversion, -bleaching (FRAP), -ablation Excitation Specs: Full set of visual cubes for blue, green, and red –emitting probes. Diode Laser Lines and Power (measured at fiber tip): 405nm, 15mw (DAPI) 488nm, 15mW (FITC, GFP) 561nm, 15mW (TRITC, Cy3, mCherry) 647nm, 15mW (far-red, Cy5) Photo-stimulation Laser 405nm, 100mW Emission Specs: Emission filters for spinning disk confocal applications: 455nm (+/- 25nm) 525nm (+/- 18nm) 605nm (+/- 35nm) 641nm (+/- 75nm) 700nm (+/- 37nm) Dual Emission: 525nm (+/- 25nm) and 646 (+/- 66nm) Objective Lenses: Plan Fluor 4x 0.13 NA WD 12.2mm Plan Apo Lambda 10x 0.45 NA WD 4.00mm Plan Apo Lambda 20x 0.75 NA WD 1.00mm Plan Fluor 40x Oil DIC H N2 1.30 NA WD 0.20mm Plan Apo Lambda Oil 60x 1.40 NA WD 0.13mm Apo TIRF Oil 100x 1.49 NA WD 0.12mm Charges: $35.00 / hour Note: To use the microscope you must have an active Account with iLab. Contact Oleg to arrange training. Location MCN T-2216 NIKON COE