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Sample Preparation

TFS Vitrobot Mark IV

By TFS - Vitrobot Mark IV

University of Nebraska-Lincoln
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The Vitrobot is a fully computer-controlled double-sided Bloating Robot for the vitrification of aqueous samples. Minimal training is required for operating the instrument in a laboratory environment. The process of plunging, blotting, and vitrification is fully automated upon placing a vial in the chamber and setting up the liquid coolant container. Operational parameters tend to be reproducible, enabling a high throughput of vitrified samples with a straightforward control of the vitrification process. The Vitrobot allows you to control the temperature, humidity, number of blotting, and several critical time settings. The instrument will consistently produce excellent specimens for Cryo-electron microscopy, including single-particle, CryoET, and MicroED.

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